Differential gene expression in wild type (RC2-wt, H99-wt) and their respective Δall1 mutants (RC2-Δall1, H99-Δall1) were confirmed by real time PCR with selected iron-related genes, Real-time PCR results on RC2-wt, RC2-Δall1
(A) and H99-wt, H99-Δall1
(B) are shown. Black bars represents genes up-regulated in mutants. Bars represent average +/− SD mRNA ratios. The mRNA levels were normalized against the respective β-actin expression. Fold expression was calculated using the delta-delta CT method.