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. Author manuscript; available in PMC: 2013 Jun 24.
Published in final edited form as: J Am Chem Soc. 2009 Nov 11;131(44):16313–16318. doi: 10.1021/ja9052398

Figure 2.

Figure 2

Folding of mini-proinsulin precursors 1 (A) and 2 (B). Conditions were: polypeptide 1 or 2, 0.2 mg mL−1; Tris: 20 mM; Cys: 8 mM; cystine: 1 mM; GnHCl 1 M; pH = 8.0. *Cys adduct, **nonpeptidic compound. (Inset) Online ESMS spectra taken at the top of the main peak in each chromatogram. (A) 1, T = 16 h; (B) 2, T = 3 h. The chromatographic separations were performed using a linear gradient (5–65%) of buffer B in buffer A over 15 min (buffer A = 0.1% TFA in water; buffer B = 0.08% TFA in acetonitrile) (UV profiles at 214 nm).