Figure 3.
Loss of hsf4 gene suppresses tumorigenesis and alters tumor spectrum in Arf-deficient mice. A, a cohort of Arf−/− (n = 15) and hsf4−/− Arf−/− (n = 19) mice was monitored for development of tumors for 59 weeks. The tumor-free survival was estimated using Kaplan–Meier survival analysis. The tumor types were determined by histologic examination and FACS analyses.*, P =0.04 was determined using log-rank (Mantel–Cox) test. B, percent tumor spectrum observed in Arf−/− and hsf4−/− Arf−/− mice. The tumors were grouped into 3 categories according to the pathologic characteristics: lymphoma/thymoma, sarcoma, and carcinoma (hepatocellular carcinoma, uterine, and colon). No tumors were found in 4 of 19 hsf4−/− Arf−/− mice during observation period of 59 weeks. C, representative histopathologic analyses using H&E staining of tumors from Arf−/− mice (a, lymphoma; c, HCC) and hsf4−/− Arf−/− (b, RMH; d, HCC) are presented. Bar = 100 μm.