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. 2013 May 28;110(25):10288–10293. doi: 10.1073/pnas.1308295110

Fig. 2.

Fig. 2.

Abundance of phospho-DNM1 in diencephalon negatively associates with the degree of arousal indexed by sleep latency. (A) Abundances of phospho-DNM1 species at ZT6, expressed relative to the average of control group. (B and C) Verification of 2D-DIGE results by Western blots. Western blots confirmed decreased DNM1 in the phosphoprotein sample from the GH group (B) but no changes in total DNM1 protein in the three groups (C). Values were normalized to β-tubulin and expressed relative to the control. (D) DNM1 mRNA levels at ZT6. The DNM1 mRNA level was measured by qPCR and normalized to Cyclophilin B mRNA level. (E) Effect of recovery sleep on phospho-DNM1 abundance as determined by 2D-DIGE. Following SD, either by GH or CC from ZT0–6, all mice were allowed to sleep freely for 2 h from ZT6–8. After 2 h of recovery sleep, phospho-DNM1 amounts in the GH group returned to baseline by ZT8. In the control and CC groups, there was not significant change in phospho-DNM1 amounts during the time course of experiments.