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. Author manuscript; available in PMC: 2013 Jun 24.
Published in final edited form as: Methods Enzymol. 2010;475:109–120. doi: 10.1016/S0076-6879(10)75005-9

Figure 5.1. Schematic of PALM analysis.

Figure 5.1

Raw data are acquired (top row) under continuous activation and excitation, then the image of each molecule is fit with a Gaussian to yield nanometric position information (bottom row). The summed information provides high-density, high-resolution molecular maps.