Neighbour-joining tree of nifH gene sequences showing the phylogenetic relationships of the root nodule bacteria isolated from Mimosa spp. Bootstrap values calculated for 1000 replications are indicated at the internodes. GenBank accession numbers are given in parentheses. The scale bar represents 2 % nucleotide substitutions per site. Abbreviations: B., Burkholderia; C., Cupriavidus; E., Ensifer; R., Rhizobium; T, type strain. Strains with the prefixes MH, MHM and MP were isolated from M. hamata, M. himalayana and M. pudica, respectively. Strains isolated in the present study are marked in bold.