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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: Bioorg Med Chem Lett. 2013 Apr 30;23(12):3481–3486. doi: 10.1016/j.bmcl.2013.04.055

Figure 2.

Figure 2

Mode of inhibition of benzobisthiazole 49. A dilution series of compound 49 was examined in the FRET-based S. aureus helicase assay20 with varying concentrations of ATP (A) or DNA (B) to determine IC50 values. Data were plotted in GraphPad Prism 5.0 using 4-parameter curve fitting. (C) The results from varying DNA are also shown in a Dixon plot (1/V vs [I]). (D) Compounds 49 and 59 failed to exhibit significant inhibition of the single-stranded DNA stimulated ATPase activity of S. aureus helicase as detected by malachite green.21