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. 2013 Jun 24;8(6):e67794. doi: 10.1371/journal.pone.0067794

Figure 10. Oxidation of various substrates by AtraAOX2.

Figure 10

(A) The oxidation of 2 mM substrates by AtraAOX2 (1 µg) was determined at 30°C and quenched with 10% acetic acid. The reduction of MTT (at 570 nm) was measured spectrophotometrically. Substrates lacking AtraAOX2 were used as blank control in order to prevent non-enzymatic background activity. To confirm AtraAOX2 activity, a zymogram was performed using 2 mM of each substrate on 7.5% Native-PAGE, respectively. (B) The oxidation of dose-dependent pesticide compounds as substrate by AtraAOX2 (1 µg) was determined as described above.