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. 2013 Jun 24;8(6):e67705. doi: 10.1371/journal.pone.0067705

Figure 5. Iph1 loss suppresses the sensitivity to proteotoxic stress oftor1 mutant in a TORC1-dependent manner.

Figure 5

A) Survival to TU (left panel) and DTT (right panel) of the indicated strains. For all strains the mean (+/− S.E.M.) of four independent experiments is shown. B) Survival to RA or TU and RA of the indicated strains. For all strains the mean (+/− S.E.M.) of three independent experiments is shown. C) Cell extracts from tor2-51 and iph1-d tor2-51 cells grown at 25°C and shifted to 36°C for 45 min were probed with anti-phospho-AKT Substrate antibodies recognizing the phosphorylated form of p27. Sty1 was detected as loading control, using anti-Hog1 antibodies. D) Survival to TU of the indicated strains. The mean (+/− S.E.M.) of four independent experiments is shown. For all experiments ANOVA with Tukey's test was applied for statistical analysis.