Skip to main content
. 2013 Jun 24;8(6):e67437. doi: 10.1371/journal.pone.0067437

Figure 4. Augmentation of HCV replication by miR-122 is not dependent on DDX6.

Figure 4

(A) Representative northern blot analysis of HCV and GAPDH RNA from cells 3 days after electroporation with J6/JFH-1 Rluc RNA and the indicated siRNA and miRNA. (B) Average band intensity measured from 3 independent northern blots as shown in part A, and (C) the corresponding fold induction of RNA accumulation by miR-122. (D) Relative luciferase expression 3 days post-electroporation in the same experiments as shown in parts A, B and C, and (E) the fold induction of luciferase expression by miR-122. (F) Relative cell numbers and (G) Fluc expression from a co-electroporated capped mRNA at 2 hour post electroporation. (H) Western blot analysis of DDX6 protein expression. Data in (D-G) represents the average of 8 independent experiments. A one-way ANOVA with Bonferonni's Multiple Comparison Test was performed on F and G to show they where not significantly different.