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. 2013 Jun 24;8(6):e67437. doi: 10.1371/journal.pone.0067437

Figure 7. miRNA translation suppression by endogenous and exogenous miRNA is alleviated by DDX6 silencing.

Figure 7

(A) Schematic diagram of the mRNAs expressed from the co-transfected reporter plasmids used in this miRNA suppression assay. Expressed mRNAs carry the Fluc sequence with 4 wild-type miR-122 binding sites in the 3′ UTR, or a control Rluc sequence. Relative Fluc:Rluc expression from the reporters was assessed in control, DDX6-depleted, and miR-122 antagonist-treated Huh7.5 cells. (B) Schematic diagram of the mRNAs expressed from the co-transfected reporter plasmids used in this miRNA suppression assay. Expressed mRNAs carry the Fluc reporter gene and 4 mutant miR-122p34 binding sites in the 3′ UTR, or a control Rluc gene. Relative Fluc:Rluc expression from the reporters was assessed in control or DDX6-depleted cells, co-transfected with the indicated amounts of miR-122p34. (C) Schematic diagram of the mRNAs expressed from the co-transfected reporter plasmids used in this miRNA suppression assay. Expressed mRNAs contain an Rluc gene with 4 miCXCR4 miRNA binding sites in the 3′ UTR or a control Fluc gene. Relative Rluc:Fluc expression from the reporters was assessed in control or DDX6-depleted cells that were co-transfected with the indicated amounts of miCXCR4. Data in A represents the average of 6 experiments and B represents the average of 4 experiments.