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. 2013 Jun 24;8(6):e67517. doi: 10.1371/journal.pone.0067517

Figure 2. Analysis of interaction of wt Lmb with human placental (HP) and EHS (Engelbroth-Holm-Swarm) tumor laminin.

Figure 2

(A) Dot blot analysis showing the interaction of wt Lmb with HP and EHS laminin. Equal concentrations (10 µg/ml) of HP and EHS laminin were spotted on a nitrocellulose membrane and probed with (10 µg/ml) wt Lmb protein. The dot blot shows that Lmb binds to both types of laminin. However, the intensity of the HP laminin spot is higher when compared with EHS Laminin spot suggesting the former has high affinity towards Lmb. (B) ELISA analysis showing the binding of wt Lmb to HP and EHS laminin. Different concentrations of wt Lmb was added to microtiter plates coated with 10 µg/ml of HP and EHS laminin and binding quantified at 405 nm. The values in the graph represent the mean+ standard deviation for the experiment in triplicates. This experiment clearly suggests that Lmb has higher affinity to HP laminin than EHS Laminin and thus supports the dot blot experiment.