Table 1. Primers used for deletion and site directed mutagenesis of Lmb and the strain designations.

Primer No.	Primer Sequence (5′-3′)	Strain designation	Mutation
1	ATT CCC GGG TTT GAC TCT ATC TAG TGT CAG	BSU 689	Δ(G123-L138)
2	ATA CCC GGG GCG ACA GTT TAT GAC CCA C
3	GCC GCG CAT ATG TGT GAT AAG TCA GCAAAC CCC A
4	GCC GCG CTC GAG CTT CAA CTG TTG ATA GAG CAC TTC C
5	GCG ACA CTT TAT GAC CCA GCT ACC TGG ACG GAT CCC	BSU 716	H142A
6	GGG ATC CGT CCA GGT AGC TGG GTC ATA AAG TGT CGC
7	GTG ACG CAA GCC ACG GCA TTT TCT TAT CTG	BSU 718	H142-206A
8	CAG ATA AGA AAA TGC CGT GGC TTG CGT CAC
9	CAA TCA GGT GCA GGC ATT GCT TCC TTT GAA CCG	BSU 778	H142-206-66A
10	CGG TTC AAA GGA AGC AAT GCC TGC ACC TGA TTG
11	ATT CCC GGG ATA CCT GGA CGG ATC CCG TTT TAG	BSU 691	DPH(140-142)-ARD
12	ATA CCC GGG CAT AAA GTG TCG CAG GGT CAA TGC
13	ATCAGGTGCAGGCATTAATTCC	BSU 702	H66N
14	GTG GTG CTC GAG CTT CAA CTG TTG ATA GAG CAC TTC C
15	ACG GTT CAA AGG AAT TAA TGC C
16	GAT ATA CAT ATG TGT GAT AAG TCA GCA AAC CCC A
11	ATT CCC GGG ATA CCT GGA CGG ATC CCG TTT TAG	BSU 704	DPH(140-142)-ARD+H66N
12	ATA CCC GGG CAT AAA GTG TCG CAG GGT CAA TGC
17	AAA GTA AAG ACA TTA AGT GCA CTT GAA GCT GCT CCA	BSU777	P279A
18	TGG AGC AGC TTC AAG TGC ACT TAA TGT CTT TAC TTT
19	GCT AAA GTA AAG ACA TTA AGT GCA CTT GAA GCT GCT CC	BSU 751	H264A
20	GG AGC AGC TTC AAG TGC ACT TAA TGT CTT TAC TTT AGC