Table 2. Metabolites significantly contributing to the separation of liver, brain and serum ¹H NMR profiles of uninfected control Balb/C mice from mice infected with P. chabaudi at peak infection or three weeks post-clearance of the parasite.

Peak Infection vs uninfected control				Three weeks post-parasite clearance vs. Uninfected controls
↑	p	↓	p	↑	p	↓	p
Liver
Male
Glucose*	−0.15	Lactate*	0.3	Glutamine#	−0.12	Glucose*	0.29
DMG*	−0.21	PCH*	0.13	L-DOPA*	−0.08
				BA*	−0.11
				Valine*	−0.11
				Isoleucine*	−0.11
				Leucine*	−0.07
				Lysine*	−0.06
				u/c*	−0.06
				2-HI*	−0.09
Females
Glutamine#	0.20	GDA*	−0.20	Glucose#	−0.15	–	–
Glutamate#	0.16	Ascorbate#	−0.19	MAA*	−0.17
L-DOPA#	0.12	MAA#	−0.15	DMG*	−0.17
Valine#	0.11	DMG#	−0.15
Isoleucine#	0.11	u/c#	−0.15
Leucine#	0.09	Histidine#	−0.16
Lysine#	0.10	Glucose#	−0.13
BA#	0.10
2-HI#	0.10
NAG#	0.09
Betaine#	0.11
Brain
Male
–	–	–	–	ACH#	0.07	–	–
Female
OAA*	0.16	–	–	PCH*	0.31	u/c*	−0.15
				ACH*	0.31	Methionine#	−0.04
Serum
Male
Glycerol*	0.07	Lipid*	−0.10	–	–	Alanine*	−0.04
		Alanine*	−0.08
		NAG*	−0.04
Female
Lactate*	0.20	Glucose*	−0.14	–	–	Creatine*	0.05

The loading weights (p) of the original variable of the PCA scores (for the relevant PC, see result) are mentioned for each metabolite. The difference across the two classes were further subjected to univariate analysis (^*p∼/<0.05, ^#P<0.005). The variations of the metabolites during 4 weeks of experiment were compared with the uninfected animals in a parallel experiment. The metabolites that did not exhibit age related changes in levels are mentioned in this table. In case of multiple NMR peaks contributing only the most significant loadings value is provided.

Keys- DMG: Dimethylglycine, PCH: Phosphorylcholine, L-DOPA: L-3,4-dihydroxyphenylalanine, BA: Bile acids, 2-HI: 2-hydroxyisovaleric acid, NAG: N-acetylglutamate, GDA: Guanidoacetate, MAA: Methylacetoacetate, ACH: Acetylcholine, OAA: Oxaloacetate, u/c: Unconfirmed.