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. 2013 Jun 24;8(6):e66883. doi: 10.1371/journal.pone.0066883

Figure 2. Analysis results for alternative splicing (AS) and differential AS (DAS) in adipose.

Figure 2

(A) Percentage of detected alterantive splicing (AS) and differential AS (DAS) events for datasets with various sequencing depths. PreLPS: detection rate for AS events in the pre-LPS sample; postLPS: detection rate for AS events in the post-LPS sample; DAS: detection rate for DAS events. (B) Spearman correlation between exon or intron inclusion levels in datasets with various sequencing depths and inclusion levels in the 500 M-read datasets. PreLPS: correlation of inclusion levels in the pre-LPS sample; postLPS: correlation of inclusion levels in the post-LPS sample; fold-change: correlation of the fold change of isoform ratios. (C) Percentage of detected AS and DAS events according to gene expression levels. preLPS_high: detection rate for AS in highly expressed genes in the pre-LPS sample; preLPS_low: detection rate for AS in lowly expressed genes in the pre-LPS sample; postLPS_high: detection rate for AS in highly expressed genes in the post-LPS sample; postLPS_low: detection rate for AS in lowly expressed genes in the post-LPS sample; DAS_high: detection rate for DAS in highly expressed genes; DAS_low: detection rate for DAS in lowly expressed genes. (D) Performance of DAS events detected in datasets with various sequencing depths.