Figure 3. Cmah−/− Mice Mount a Greater CD8 T Cell Response to AAV after Ad-AAV Immunization.

Mice were immunized with human Adenovirus serotype 5 intramuscularly. (A) Representative frequency of H-2K^b AAV2-Pentamer+ CD8+ T cells in the blood at baseline (top) and day 10 (bottom) of WT (left panels) and Cmah−/− (middle panels) mice and the number of H-2K^b AAV2-Pentamer+ CD8+ T cells per IE6 lymphocytes (right panels). (B) Representative frequency of H-2K^b AAV2-Pentamer+ CD8+ T cells in the spleen at day 10 of WT (left panel) and Cmah−/− (middle left panel) mice and the frequency of H-2K^b AAV2-Pentamer+ CD8+ T cells (middle right panel). Absolute numbers of H-2K^b AAV2-Pentamer+ CD8+ T cells is plotted in the far right panel. (C) Representative Frequency of IFNγ+ CD8 T Cells in day 10 spleens of WT (left panel) and Cmah−/− (middle left panel) mice after 5 hours of H-2K^b AAV2 peptide SNYNKSVNV stimulation, and the summary plot of all frequencies in each group (middle right panel). Absolute numbers of IFNγ+ CD8 T cells is plotted in the far right panel. *p<0.05, #p<0.1, student’s t test. N=5 mice per group. Results are representative of at least two experiments.