Figure 4. Cmah−/− Mice Mount a Greater T Cell Response During Acute LCMV Armstrong Infection.

Mice were infected with acute LCMV strain Armstrong and bled at various time points until sacrifice and spleen isolation at day 42. (A–C) Mouse PBMC. (A) Frequency of CD8+ T cells at day 8 in the blood of WT (left plot and black triangles in right plot) and Cmah−/− (middle plot and red squares in right plot) mice. Left and middle plots show the median representative dot plots from each group, while the plot on the right shows quantification of the frequencies. (B) Number of LCMV H2-D^b gp33 tetramer+ CD8+ T cells per 1E6 lymphocytes in the blood of WT (black line, right plot) and Cmah−/− (red line, right plot) mice over 42 days. Left and middle plots show the median representative dot plots from day 42 from each group, while the right plot graphs the number of tetramer+ cells per 1E6 lymphocytes over time. (C) Number of LCMV H2-D^b gp33 tetramer+ CD8+ T cells that are KLRG1-CD127+ per 1E6 lymphocytes in the blood of WT (black line, right plot) and Cmah−/− (red line, right plot) mice over 42 days. Left and middle plots show the median representative dot plots from day 42 from each group, while the right plot graphs the number of memory-phenotype tetramer+ cells per 1E6 lymphocytes over time. (D and E) Mouse splenocytes at day 42. (D) Left and middle plots show the median representative dot plots of the frequencies of LCMV H2-D^b gp33 tetramer+ CD8+ splenocytes from day 42 from each group, while the plot on the right shows quantification of the absolute numbers of these cells in the spleen. WT (black triangles in right plot) and Cmah−/− (red squares in right plot). Similar trends were observed in the number of gp276 tetramer+ CD8+ splenocytes. (E) Left and middle plots show the median representative dot plots of the frequencies of KLRG1-CD127+ LCMV H2-D^b gp33 tetramer+ CD8+ splenocytes, while the plot on the right shows quantification of these frequencies in WT (black triangles in right plot) and Cmah−/− (red squares in right plot) spleens. (F) Day 7 CD8 donor (Ly5.2) analysis in Ly5.1 recipient mice infected with LCMV. Left plot shows the number of gp33+ CD8+ donor T cells per spleen, and right plot shows the number of gp33+ CD8+ donor T cells per 1E6 lymphocytes in the blood. Cmah−/− (red squares, 4 mice), WT (black triangles, 3 mice). *p<0.05, #p<0.1, student’s t test. N=5 mice per group (A–E). Results are representative of at least two experiments.