Upregulation of NKG2D ligand and cytokine expression is dependent on STAT1 signaling in DCs. CD11c+ DCs generated from the bone marrow of WT and STAT1−/− mice were stimulated for 24 h with live VV, IFN-α, or left untreated (Ctrl). Thereafter, RNA was purified and the expression of NKG2D ligands, Rae-1 and Mult1 (A), and IL-15 and IL-12 (B) was assayed by quantitative real-time PCR. The mean fold increase ± SD of expression over control (Ctrl) is provided (n = 3). mRNA abundance was normalized to β-actin.