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. 2013 Jun 24;8(6):e67589. doi: 10.1371/journal.pone.0067589

Figure 2. The GSTP1 Ile105Val polymorphism affects breast cancer cell response to cyclophosphamide.

Figure 2

(A) The GSTP1 mRNA expression levels in breast cancer cell lines were detected using RT-QPCR. (B) The GSTP1 protein expression levels in breast cancer cell lines were detected using western blot. (C) MDA-MB-435 (Ile/Ile), MCF-7 (Ile/Val), and T47D (Val/Val) cells lines were treated with 4-HC (15 µg/mL) for indicated time, and cell survival rates were analyzed using MTT assay at the indicated time points. (D) MCF-7 cells transfected with pEGFP (Vector), pEGFP-GSTP1 105Ile (Ile/Ile), pEGFP-GSTP1 105Val (Val/Val) were treated with 4-HC (15 µg/mL) for indicated time, and cell survival rates were analyzed using MTT assay at the indicated time points. The cells were tested in three independent assays with each containing triplicates and the calculated data of experiments are expressed as mean ± SD.