Table 4. Gene expression of selected genes in liver of rats after 50 weeks of diet administration¹.

	Dietary supplementation2				Statistical significance of variance ratio (P)3, effects of
	Control	TTA	FO	TTA+FO	TTA	FO	TTA*FO
Aldh9a1	1.00±0.20	1.31±0.18	1.02±0.61	1.11±0.16	0.07	0.40	0.31
Bbox1	1.00±0.27	1.13±0.21	0.86±0.27	1.05±0.26	0.05	0.18	0.65
Tmlhe	1.00±0.22	1.15±0.13	0.98±0.26	1.02±0.13	0.15	0.26	0.34
Aox3	1.00±0.22	0.31±0.16	0.83±0.47	0.33±0.15	<0.001	0.38	0.30
Clpx	1.00±0.33	2.76±0.87	1.48±0.42	2.81±0.73	<0.001	0.20	0.29
Cyp7b1	1.00±0.31	0.97±0.34	0.93±0.34	0.69±0.14	0.17	0.11	0.28
Aadat	1.00±0.20	1.41±0.23	0.96±0.25	1.45±0.36	<0.001	0.98	0.65
Glyctk	1.00±0.19	1.21±0.19	0.98±0.22	1.04±0.11	0.03	0.10	0.17
Cbs	1.00±0.31	1.03±0.38	0.78±0.24	1.16±0.21	0.03	0.66	0.07
Shmt2	1.00±0.31	1.31±0.38	0.84±0.22	1.19±0.44	0.005	0.19	0.85
Hdc	1.00±0.70	57.83±20.87	10.43±3.91	71.74±30.43	<0.001	0.05	0.69
Glul	1.00±0.43	1.03±0.34	0.90±0.26	1.12±0.38	0.28	0.93	0.38
Glud1	1.00±0.27	1.57±0.28	0.94±0.37	1.40±0.34	<0.001	0.24	0.60
Arg1	1.00±0.38	0.86±0.23	0.58±0.17	0.69±0.23	0.88	0.001	0.13
Ass1	1.00±0.43	0.41±0.19	0.57±0.39	0.44±0.16	0.001	0.06	0.03
Asl	1.00±0.28	0.59±0.15	0.78±0.24	0.61±0.10	<0.001	0.11	0.07

Abbreviations: TTA, tetradecylthioacetic acid; FO, fish oil; Aldh9a1, aldehyde dehydrogenase 9, subfamily A1; Bbox1, gamma-butyrobetaine hydroxylase 1; Tmlhe, trimethyllysine hydroxylase, epsilon; Aox3, aldehyde oxidase 3; Clpx, ClpX caseinolytic peptidase×homolog; Cyp7b1, cytochrome P450, family 7, subfamily B, polypeptide 1; Aadat, aminoadipate aminotransferase; Glyctk, glycerate kinase; Cbs, cystathionine beta synthase; Shmt2, serine hydroxymethyltransferase; Hdc, histidine decarboxylase; Glul, glutamate-ammonia ligase; Glud1, glutamate dehydrogenase 1; Arg1, arginase, liver; Ass1, argininosuccinate synthase 1; Asl, argininosuccinate lyase.

¹Relative to control diet.

²Values are mean ± SD (n = 10).

³P-values from two-way ANOVA.