Table II.
Smell impaired P[GT1] insertion lines.
|
P-element line |
Disrupted locus | P[GT1] insertion site | Mutational effect (/ΔLRI/) |
Smell impaired for adult OB? | |
|---|---|---|---|---|---|
| Propionic acid |
|||||
| BG00737 | Hsp27 | In 5′-UTR and exon 1 | 24.44* | NO | |
| BG01011 | Spinophilin (Spn) /misshapen (msn) | 55 bp upstream 5′ region /1443 bp downstream 3′ region |
16** | YES (Sambandan et al. 2006) | |
| BG01179 | defense repressor 1 (dnr1) | In intron | 39.69*** | NO | |
| BG01223 | Glutamine synthetase 2 (Gs2) | In intron | 6.5* | NO | |
| BG01228 | derailed (drl) | 22,8 kbp downstream 3′ region | 12.24* | YES (Moreau-Fauvarque et al. 2002) | |
| BG01257 | jing | 290 bp downstream 5′ region | 28.54** | YES (Sambandan et al. 2006) | |
| BG01330 | CG11883 | In intron | 11 4*** | NO | |
| BG01376 | Chd64 | In 5′-UTR | 9.4** | NO | |
| BG01380 | Oseg4 / draper (drpr) | In exon 1 / In 5′-UTR | 9.42** | NO / YES (MacDonald et al. 2006) | |
| BG01399 | CG17646 | In intron | 7.5* | NO | |
| BG01404 | bicoid-interacting protein 3 (bin3) | In intron | 31.1** | YES (Sambandan et al. 2006) | |
| BG01515 | female sterile (1) homeotic (fs(1)h) | In exons 19 and 20, also in introns | 28.97** | NO | |
| BG01683 | CG32572 | In intron | 39.2*** | NO | |
| BG01733 | CG6175 | 2725 bp upstream 5′ region | 38.7*** | NO | |
| BG02042 | easily shocked (eas) | In exon 2 | 32.5* | NO | |
| BG02081 | Rtnl1 | In 5′-UTR, exon 10 and intron | 27.3* | YES (Sambandan et al. 2006) | |
| BG02823 | scylla (scyl) | 130 bp downstream 5′ region | 35.7* | NO | |
|
| |||||
| Benzaldehyde | |||||
| BG01735 | CG13130 / big brain (bib) | In exon 2 / In 5′-UTR and exon 1 | 20.25* | NO / NO | |
| BG01897 | jing | 143 bp upstream 5′ region | 21* | YES (Sambandan et al. 2006) | |
| BG02102 | Quasimodo † | 2,15 kbp upstream 5′ region | 20.8* | NO | |
|
| |||||
| Nonanol | |||||
| BG00737 | Hsp27 | In 5′-UTR and exon 1 | 29.3* | NO | |
| BG01043 | Gp150 | In 5′-UTR and exons 1, 7 and 8 | 20.9* | NO | |
| BG01173 | CG5361 | 11,8 kbp upstream 5′ region | 32** | NO | |
| BG01315 | couch potato (cpo) | In intron | 23.8* | YES (Sambandan et al. 2006) | |
| BG01324 | cricklet (clt) | In 5′-UTR and exon 4 | 33.2** | NO | |
| BG01600 |
Chronologically inappropriate
morphogenesis (chinmo) |
1244 bp upstream 5′ region | 38.6* | NO | |
| BG01683 | CG32572 | In intron | 25.4* | NO | |
| BG01733 | CG6175 | 2725 bp upstream 5′ region | 31.9* | NO | |
Lines and/or genes that showed a smell impaired mutant phenotype in response to more than one odorant are in bold. Candidate genes used for further characterization of expression patterns are underlined. Mutational effects were calculated as the absolute values of the difference between the LRI of the smell impaired P[GT1] line versus the isogenic control line. Significant deviations from the control line using a Dunnett’s test are indicated by asterisks.
: p < 0.05
: p < 0.01
: p < 0.001.
Information on P[GT1] insertion site and gene name was obtained from FlyBase (flybase.org). Genes categorization as “smell impaired for adult OB?” was based on the previous works cited in each case.
also known as l(2)05510.