Fig. 3. Effects of Pertussis toxin and La³⁺ on C3a, hBD2 and hBD3-induced Ca²⁺ mobilization and degranulation in human mast cells.

(A, E) Indo-1 loaded LAD2 cells were exposed to C3a, followed by hBD2 or hBD3 and intracellular Ca²⁺ mobilization was determined. (B, F), Cells were treated with medium or pertussis toxin (PTx; 100 ng/ml, 16 h) and effects of C3a, hBD2 or hBD3 on Ca²⁺ mobilization was determined. (C, G), Indo-1 loaded LAD2 cells were exposed to La³⁺ (lanthanum chloride, 1 μM) and C3a, hBD2 or hBD3-induced Ca²⁺ mobilization was determined. Traces are representative of 3 independent experiments. LAD2 mast cells were exposed to (D), Pertussis toxin (PTx; 100 ng/ml, 16 h) or (H) La³⁺ (lanthanum chloride, 1 μM, 30 min) and C3a, hBD2 and hBD3-induced degranulation was determined. Data are mean ± SEM of three experiments. Statistical significance was determined by two-way ANOVA with Bonferroni's post test. ** indicates p<0.001.