Skip to main content
. 2013 Jun 25;3:2069. doi: 10.1038/srep02069

Figure 4. Roles of the dally-mediated pathways in phagocytosis.

Figure 4

(A) The expression profiles of genes belonging to the dally-mediated pathways in S2 cells in response to LPS or PG challenge. The rp49 gene was used as a control. Statistically significant differences between treatments were indicated with asterisks (** P < 0.01). Control, non-treated S2 cells. (B) The expression profiles of other genes belonging to the Wnt pathways in S2 cells in response to LPS or PG challenge. (C) Effects of dally gene silencing on gene expressions of the Wnt pathway. To evaluate the involvement of dally in phagocytosis, expression of the dally gene in S2 cells was silenced using sequence-specific siRNA. The expression profiles of the genes related to the dally-mediated pathways were then examined using quantitative RT-PCR. The rp49 gene was used as a control. Statistically significant differences between treatments were indicated with asterisks (*P < 0.01 or **P < 0.01). Control, non-treated S2 cells. (D) Effects of dally gene silencing on expressions of genes belonging to the dally-mediated pathways. (E) Percentages of S2 phagocytosis against the pHrodo-labeled WSSV virions. The number of S2 cells phagocytosing the pHrodo-labeled WSSV virions was quantified using flow cytometry at 1 hr after virus inoculation. The inactivated WSSV (WSSV) and non-inactivated WSSV [WSSV (live)] were included in the experiments. Non-treated S2 cell was used as a control. Statistically significant differences between treatments were indicated with asterisks (** P < 0.01). (F) The model of the dally-mediated pathways involved in phagocytosis. The Wnt pathway was shown in the box.