Table 1.
Differentially expressed HCC, fibrotic liver nuclear membrane proteins and HepG2 cell line
| Acc | Name | Abb | Thr. pI; mass | Score | Peptide matched | Cellular compartment | PTM | %age cov | Mean normalized volume fibrosis HCC /HepG2 | Ratio | Anova (P) | Fold change | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| |
|
|
|
|
|
CF(1), Membrane |
|
|
|
|
|
|
|
|
P30049 |
ATP synthase subunit delta |
ATPD |
5.38; 17 |
58 |
5 |
mitochondrion |
Isopeptide bond |
17% |
0.02536 |
0.00704 |
0.277 |
0.02 |
3.6 |
| Mitochondrion |
Ubl conjugation |
|
|
|
|
|
|
||||||
| inner membrane |
|
|
|
|
|
|
|
||||||
|
P02675 |
Fibrinogen beta chain |
FIBB |
8.54; 55 |
1009 |
27 |
Secreted |
Disulfide bond |
41% |
0.03364 |
0.01628 |
0.483 |
0.05 |
2.1 |
| Glycoprotein |
|
|
|
|
|
|
|||||||
| Pyrrolidone |
|
|
|
|
|
|
|||||||
| carboxylic acid |
|
|
|
|
|
|
|||||||
|
P06576 |
ATP synthase subunit beta |
ATPB |
5.26; 56 |
670 |
18 |
CF(1), Membrane |
Acetylation |
29% |
0.03448 |
0.00913 |
0.264 |
0.04 |
3.7 |
| mitochondrion |
Phosphoprotein |
|
|
|
|
|
|
||||||
| Mitochondrion inner membrane |
|
|
|
|
|
|
|
||||||
|
Q549N7 |
Hemoglobin subunit beta |
HBB |
6.75;15 |
484 |
22 |
Heptoglobin- |
Acetylation |
61% |
0.22697 |
0.11803 |
0.520 |
0.01 |
1.9 |
| hemoglobin |
Glycation |
|
|
|
|
|
|
||||||
| complex |
Glycoprotein |
||||||||||||
| |
Phosphoprotein |
||||||||||||
| |
S-nitrosylation |
||||||||||||
|
P00167 |
Cytochrome b5 OS |
CYB5A |
4.88; 15 |
176 |
11 |
Cytoplasmic |
Acetylation |
42% |
0.01948 |
0.05883 |
3.020 |
0.04 |
3.0 |
| Endoplasmic |
|
|
|
|
|
|
|
||||||
| reticulum | |||||||||||||
| Membrane | |||||||||||||
| Microsome | |||||||||||||
| P31930 | Cytochrome b-c1 complex subunit 1 | QCR1 | 5.94; 52 | 149 | 10 | Mitochondrion |
Acetylation |
15% |
0.01607 |
0.00505 |
0.314 |
0.05 |
3.1 |
| inner membrane | Phosphoprotein | ||||||||||||
Accession no. is obtained from SWISS/Prot and percent coverage refers to the percentage of protein sequence coverage, determined by number of matched peptides. Significance was calculated from the Progenesis SameSpots v4.5 (Nonlinear Dynamic, UK), generated statistical analysis. A value of p < 0.05 was considered statistically significant. Functional classes were determined by searching the UniProt database (http://www.uniprot.org).
The proteins were separated on 2DE gels and identified by ESI-QTOF MS/MS.