Table 2.
Stratification and data distribution of the investigated experimental factors
| Factor | No. of embryo transfers (%) | |
|---|---|---|
|
Season1 |
|
|
| - Spring |
39 |
(20.2) |
| - Summer |
59 |
(30.6) |
| - Autumn |
58 |
(30.0) |
| - Winter |
37 |
(19.2) |
|
Type of genetic modification2 |
|
|
| - de novo - AGT |
57 |
(29.5) |
| - HR |
48 |
(24.9) |
| - replication of transgenic pig |
88 |
(45.6) |
|
Donor cell source3 |
|
|
| - MSC |
36 |
(18.7) |
| - PF |
24 |
(12.4) |
| - FF |
51 |
(26.4) |
| - KC |
82 |
(42.5) |
|
Cloning rounds |
|
|
| - 1 time |
110 |
(57.0) |
| - 2 times |
62 |
(32.1) |
| - 3 times |
21 |
(10.9) |
|
Selection of SCNT embryos for early development4 |
|
|
| - no selection |
45 |
(23.3) |
| - selection after 1 day |
13 |
(6.7) |
| - selection after 2 days |
15 |
(7.8) |
| - mixed selection | 120 | (62.2) |
Additional file 3 and Additional file 4 show in more detail the distribution in season and embryo selection of specific SCNT configurations with respect to genetic modification, donor cell source and number of cloning rounds.
1 Embryo transfer date.
2 AGT: additive gene transfer, HR: homologous recombination.
3 Mesenchymal stem cells (MSC), postnatal fibroblasts (PF), fetal fibroblasts (FF), and kidney cells (KC).
4 No selection: all SCNT embryos transferred, selection for 1 day: 1-cell stage SCNT embryos transferred, selection for 2 days: 2-cell to 4-cell stage SCNT embryos transferred, mixed selection: mixed SCNT embryos transferred (no selection/1 day and 1 day/2 days).