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. Author manuscript; available in PMC: 2013 Oct 31.
Published in final edited form as: J Immunol Methods. 2012 Jun 28;384(0):1–9. doi: 10.1016/j.jim.2012.06.010

Fig. 3.

Fig. 3

Inhibition ELISA demonstrated the absence of cross-reactivity between MAb 23C8 and MAb 16F4. Pre-incubation with different concentration of MAb 16F4 did not inhibit HRP-conjugated MAb 23C8 binding to NAP-26695 antigen (A). Similar results were obtained after pre-incubation with MAb 23C8 and HRP-conjugated MAb 16F4 (B). In contrast HRP-conjugated antibody binding was inhibited when the same MAb was used for pre-incubation. Standard curves from representative capture ELISA tests using bacterial derived recombinant NAP-26695 (C) or lambda-NAP from MV-lambda-NAP-infected Vero cells (D) as assay standards, demonstrate the higher sensitivity of the assay variant with mammalian cell expressed lambda-NAP standard.