Table 1.
Sequences of oligonucleotide primers used to amplify 10 microsatellite loci of Plasmodium falciparum and virtual heterozygosity (HE) and number of alleles observed in 164 isolates from western Cambodia
| Namea | 5′ Label | Sequence 5′-3′ | Chromosome | Virtual heterozygosity (HE) |
No. of alleles |
|---|---|---|---|---|---|
| Polyα-F | AAAATATAGACGAACAGA | 4 | 0.882 | 17 | |
| Polyα-R | VIC | ATCAGATAATTGTTGGTA | c | ||
| TA60-F | VIC | CTCAAAGAAAAATAATTCA | 13 | 0.722 | 15 |
| TA60-R | AAAAAGGAGGATAAATACAT | ||||
| ARA2-F | NED | GTACATATGAATCACCAA | 11 | 0.698 | 10 |
| ARA2-R | GCIII GAGTATTATTAATA | ||||
| PfG377-F | GATCTCAACGGAAATTAT | 12 | 0.587 | 6 | |
| PfG377-R | NED | TTATGTTGGTACCGTGT | |||
| PfPK2-F | CTTTCATCGATACTACGA | 12 | 0.840 | 20 | |
| PfPK2-R | NED | CCTCAGACTGAAATGCAT | |||
| TA87-F | VIC | ATGGGTTAAATGAGGTACA | 6 | 0.747 | 11 |
| TA87-R | ACATGTTCATATTACTCAC | ||||
| TA109-F | FAM | TAGGGAACATCATAAGGAT | 6 | 0.345 | 9 |
| TA109-R | CCTATACCAAACATGCTAAA | ||||
| C2M3-F | NED | GGTTAATATGATCACAAAATG | 2 | 0.508 | 3 |
| C2M3-R | ATTGTTGATTCATGAAATGCA | c | |||
| TA81-F | FAM | GAAGAAATAAGGGAAGGT | 5 | 0.807 | 9 |
| TA81-R | TTTCACACAACACAGGATT | ||||
| TA42-F | VIC | ACAAAAGGGTGGTGATTCT | 5 | 0.466 | 12 |
| TA42-R | GTATTATTACTACTACTAAAG |
a
F = forward, R = reverse. The fluorescent dyes used to label primers were VIC (Applied Biosystems proprietary “green” fluorescent dye), NED (Applied Biosystems proprietary “yellow” fluorescent dye), and 6-FAM (6-carboxyfluorescein, “blue” fluorescent dye).