Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Properties of fluorescent dyes. (a) Spectral properties of dyes. (b) Normalized absorbance at λmax versus pH for fluorescein (1) and carbofluorescein (4). Error bars show standard error (SE; n = 2). Determined values (±SE): compound 1, pKa = 6.35 ± 0.02, Hill coefficient = 0.93 ± 0.02; compound 4, pKa = 7.44 ± 0.01, Hill coefficient = 1.33 ± 0.03. (c) Absorption at λmax versus dielectric constant for rhodamine 110 (2) and carborhodamine 110 (5). Error bars show SE (n = 2).
