Skip to main content
. 2013 Jun 17;9:118. doi: 10.1186/1746-6148-9-118

Table 5.

Primer pairs sequences for quantitative real-time PCR1-4

Gene symbol Full gene name Acc. number 1 Primer pairs (5’-3’)2 Exons spanned Product size (bp)
ACACA
Acetyl-CoA carboxylase alpha
NM_174224
F: ttc acg tgg cct ggg tag a
40-41
142
 
 
 
R: ttg tac ctg gat tct cct tca tct t
 
 
CPT1B
Carnitine palmitoyltransferase 1B
NM_001034349
F: gcg act cca gtg gga cat tc
12-13
114
 
 
 
R: aaa ggc agg aac tgg aag ca
 
 
CRAT
Carnitine O-acetyltransferase
NM_001075587
F: ggc cca ccg agc cta cac
12-13
138
 
 
 
R: atg gca atg gcg tag gag gt
 
 
FABP4
Fatty acid-binding protein 4
NM_174314
F: tgg atg ata aga tgg tgc tgg a
3-4
114
 
 
 
R: atg gag ttc gat gca aac gtc
 
 
LPL
Lipoprotein lipase
NM_001075120
F: act gtg gct gag agc gag aac
7-8
98
 
 
 
R: tct cca ata tcc acc tcc gtg ta
 
 
PPARA
Peroxisome proliferator-activated receptor alpha
NM_001034036.1
F: agt gcc ttt cag ttg gga tgt c
2-3
125
 
 
 
R: cgc ggt ttc gga atc ttc ta
 
 
PPARG
Peroxisome proliferator-activated receptor gamma
NM_181024
F: tgt ctc ata atg cca tca ggt ttg
4-5
66
 
 
 
R: tct ccg cta aca gct tct cct t
 
 
SCD
Stearoyl-CoA desaturase
NM_173959
F: cca tca acc ccc gag aga at
5-6
76
 
 
 
R: aag gtg tgg tgg tag ttg tgg aa
 
 
SREBF1
Sterol regulatory element binding transcription factor 1
NM_001113302
F: agc ctg gca atg tgt gag aag
13-14
115
 
 
 
R: caa gga gca ggt cac aca gga
 
 
PPIB3
Peptidylprolyl isomerase B
NM_174152
F: tcc gtc ttc ttc ctg ctg ttg
1-2
98
R: cca att cgc agg tca aag tac
RPLP04
Ribosomal protein P0
NM_001012682
F: gca ttc ccg ctt cct gg
5-6
109
      R: gcg ctt gta ccc att gat ga    

§Published in [69].

1Entrez Gene, National Center for Biotechnology Information (NCBI).

2F: forward primer and R: reverse primer.

3Housekeeping gene for muscle.

4Housekeeping gene for subcutaneous adipose tissue.