Table 1.
Specific shRNA Introduced by HphA Inhibits the Expression of HCV 5′-NTR and Core Genes
| Luciferase activity (RFU) | Blank | Mock | Psh-72 | Psh-274 | Psh-365 | Psh-1019 | Scrambled |
|---|---|---|---|---|---|---|---|
| With FCS (10%) in medium | 0 | 6154 5224 6157 |
2095 3305 2608 |
2458 1987 2509 |
3257 1926 2425 |
2982 2334 731 |
5471 6234 5012 |
| 5845±414 | 2269±424* | 2318±221* | 2536±481* | 2016±856* | 5572±441 | ||
| 59% | 59% | 55% | 64% | ||||
| Without FCS in medium | 0 | 5843 5709 5324 |
2178 2357 2987 |
2475 2067 2378 |
2878 2390 2641 |
2309 2256 1668 |
5617 6146 5987 |
| 5625±200 | 2507±319* | 2307±160* | 2635±164* | 2077±273* | 5917±199 | ||
| Inhibition rate | 58% | 62% | 54% | 65% |
* p<0.05 compared with luciferase activity in the Mock group in the same FCS medium