Table 4.
Primer sequences for detection of PCVs and TTSuVs
| Primer | Primer sequence | Location (nt) | Product size (bp) |
|---|---|---|---|
| PTTV1-F1 |
5’-CGGGTTCAGGAGGCTCAAT-3’ |
8–26 |
305 |
| PTTV1-R1 |
5’-GCCATTCGGAACTGCACTTACT-3’ |
291–312 |
|
| PTTV2-F1 |
5’-TCATGACAGGGTTCACCGGA-3’ |
1–20 |
252 |
| PTTV2-R1 |
5’-CGTCTGCGCACTTACTTATATACTCTA-3’ |
226–252 |
|
| PTTV1-F2 |
CAGCGGTAGACAGAACTGTCTAGCGACT |
328–355 |
< 3000 |
| PTTV1-R2 |
TCTGTCTACCGCTGGCGGCATAAACTCA |
314–341 |
|
| PTTV2-F2 |
TCGAGCTCCTGAGAGCGGAGTCAAGGGGCCTA |
322–353 |
< 3000 |
| PTTV2-R2 |
TCGAGCTCCGGCACCCGCCCAGGCGGTTAGAC |
300–331 |
|
| PCV1-F1 |
5’-TTGCTGAGCCTAGCGACACC-3’ |
596–615 |
350 |
| PCV1-R1 |
5’-TCCACCTGCTTTCAAATCGGCC-3’ |
924–945 |
|
| PCV2-F1 |
5’-CAGCAAGAAGAATGGAAGAAGCGGA-3’ |
56–80 |
1057 |
| PCV2-R1 | 5’-CCAGGACTACAATATCCGTGTAACT-3’ | 1088–1112 |