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(A) HPLC chromatogram of the crude glutaredoxin peptide preparation containing MeCys. The major peak (labeled) is the desired oxidized peptide. The high purity of the product is evident from the chromatogram. The dark line is the absorbance at 214 nm and the grey dashed line is the absorbance at 254 nm. (B) MALDI-TOF mass spectral chromatogram of the desired oxidized peptide.
