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Evolution of fluorescence (365/440 nm) following addition of β-gal (10 units, E801A) to solutions of 19-23 (10mM) in PBS (1.0mL, 0.1M, pH=7.4) at 20-22°C showing release of the corresponding aglycones 19→1(*), 20→2(□), 21→3(♦), 22→4(Δ), 23→5(○).
