Fig. 3.

Deletion of ROCK1 inhibits upregulation of renal TGF-β₁, IL-1β and renal fibrosis in a mouse model of diabetes. a, b TGF-β₁ mRNA and protein expression detected by Western blot and real-time PCR. c IL-1β mRNA expression by real-time PCR. d Renal fibrosis examined by picrosirius red and fast green staining. Results show that deletion of ROCK1 inhibits upregulation of renal TGF-β₁, IL-1β, and the development of tubulointerstitial fibrosis in the diabetic kidney at 8 weeks after STZ treatment. Each bar represents mean ± SEM for a group of 6 mice. * p < 0.05, ** p < 0.01 compared to control mice; ^# p < 0.05, ^## p < 0.01 compared to WT mice treated with STZ. ×400.