Table 1.
Ingredient | Control diet (g/kg) | ABA diet (g/kg) |
---|---|---|
Casein | 200 | 200 |
L-cystine | 3 | 3 |
Corn Starch | 397.5 | 397.5 |
Maltodextrin | 132 | 132 |
Sucrose | 100 | 100 |
Cellulose | 50 | 50 |
Mineral mix (AIN-93) 1 | 35 | 35 |
Vitamin mix (AIN-93) 2 | 10 | 10 |
Choline bitartrate | 2.5 | 2.5 |
Tert-
butylhydroquinone 3 |
0.014 | 0.014 |
Soybean Oil | 70 | 70 |
Abscisic acid 3 | 0 | 0.1 |
Per kg of diet: 3 g nicotinic acid, 1.6 g calcium pantotenate, 0.7 g pyridoxine HCl, 0.6 g thiamin HCl, 0.6 g riboflavin, 0.2 g folic acid, 0.02 g D-biotin, 2.5 g vitamin B12 (0.1% in mannitol), 15 g d,l-α tocopheryl acetate (500 IU/g), 0.8 g vitamin A palmitate (500,000 IU/g), 0.2 g vitamin D3 (cholecalciferol, 500,000 IU/g), 0.075 g vitamin K (phylloquinone), and 974.705 g sucrose.
Per kg of diet: 357 g calcium carbonate, 196 g potassium phosphate monobasic, 70.78 g potassium citrate, 74 g sodium chloride, 46.6 g potassium sulfate, 24.3 g magnesium oxide, 6.06 g ferric citrate, 1.65 g zinc carbonate, 0.63 g manganous carbonate, 0.31 g cupric carbonate, 0.01 g potassium iodate, 0.01025 g sodium selenate, 0.00795 g ammonium paramolybdate, 1.45 g sodium meta-silicate, 0.275 g chromium potassium
Abscisic acid (ABA), or (2-cis,4-trans)-5-(1-Hydroxy-2,6,6-trimethyl-4-oxo-2-cyclohexen-1-yl)-3-methyl-2,4-pentadienoic acid, was added as a 50:50 mixture of the (S)-5 and (R)-5 isomers (Sigma).