Skip to main content
. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: ESPEN J. 2012 Aug 2;7(5):e189–e195. doi: 10.1016/j.clnme.2012.07.002

Table 1.

Composition of Experimental Diets

Ingredient Control diet (g/kg) ABA diet (g/kg)
Casein 200 200
L-cystine 3 3
Corn Starch 397.5 397.5
Maltodextrin 132 132
Sucrose 100 100
Cellulose 50 50
Mineral mix (AIN-93) 1 35 35
Vitamin mix (AIN-93) 2 10 10
Choline bitartrate 2.5 2.5
Tert-
butylhydroquinone 3
0.014 0.014
Soybean Oil 70 70
Abscisic acid 3 0 0.1
1

Per kg of diet: 3 g nicotinic acid, 1.6 g calcium pantotenate, 0.7 g pyridoxine HCl, 0.6 g thiamin HCl, 0.6 g riboflavin, 0.2 g folic acid, 0.02 g D-biotin, 2.5 g vitamin B12 (0.1% in mannitol), 15 g d,l-α tocopheryl acetate (500 IU/g), 0.8 g vitamin A palmitate (500,000 IU/g), 0.2 g vitamin D3 (cholecalciferol, 500,000 IU/g), 0.075 g vitamin K (phylloquinone), and 974.705 g sucrose.

2

Per kg of diet: 357 g calcium carbonate, 196 g potassium phosphate monobasic, 70.78 g potassium citrate, 74 g sodium chloride, 46.6 g potassium sulfate, 24.3 g magnesium oxide, 6.06 g ferric citrate, 1.65 g zinc carbonate, 0.63 g manganous carbonate, 0.31 g cupric carbonate, 0.01 g potassium iodate, 0.01025 g sodium selenate, 0.00795 g ammonium paramolybdate, 1.45 g sodium meta-silicate, 0.275 g chromium potassium

3

Abscisic acid (ABA), or (2-cis,4-trans)-5-(1-Hydroxy-2,6,6-trimethyl-4-oxo-2-cyclohexen-1-yl)-3-methyl-2,4-pentadienoic acid, was added as a 50:50 mixture of the (S)-5 and (R)-5 isomers (Sigma).