Table 2. Φbinding values for the interaction between wild type and mutants of ACTRWT and NCBDY2108W, respectively, measured in 20 mM phosphate (pH = 7.4), 150 mM NaCl, 0.7 M TMAO, and 277 K.
| ACTRWT | NCBDY2108W | ||||
|---|---|---|---|---|---|
| NCBDY2108W mutant | ΔΔGEq kcal/mol | Φbinding | ACTR mutant | ΔΔGEq kcal/mol | Φbinding |
| L2067A α1 | 0.63 ± 0.08 | 0.0 ± 0.1 | L1064A α2 | 2.5 ± 0.1 | 0.06 ± 0.02 |
| L2070A α1 | 1.2 ± 0.1 | 0.20 ± 0.04 | L1071A loop/α2 | 3.1 ± 0.2 | 0.14 ± 0.03 |
| L2074A α1 | 1.3 ± 0.1 | 0.19 ± 0.04 | |||
| A2099G α3 | 1.2 ± 0.1 | 0.23 ± 0.04 | |||