Figure 3.

Blockade of nerve injury-induced cPLA₂ and iPLA₂ activations. (a and b) Activation of spinal cPLA₂ (panel a) and iPLA₂ (panel b) were detected by cPLA₂ and iPLA₂ activity assays at defined time points after nerve injury. The capital letter “C” represents the control group (naive mice). (c and d) After pre-treatments of vehicle, MK-801, CP-99994, AACOCF3, BEL (each 10 nmol, i.t.) and minocycline (30 mg/ml, i.p.) before nerve injury, the activities of spinal cPLA2 (panel c) and iPLA2 (panel d) at 1 h after injury were evaluated. The “veh”, “MK”, “CP”, “AA” and “mino” represent vehicle, MK-801, CP-99994, AACOCF3 and minocycline, respectively. (e and f) Activities of cPLA₂(panel e) and iPLA₂ (panel f) were measured at 1 h after injury using the Lpar1- and Lpar3-deficient mice. “WT” represents the wide-type mice. (g) PLA₁ activity in the spinal dorsal horn was measured by PLA₁ activity assay at time-course points after nerve injury. Data represent means ± SEM from experiments using 3-6 mice. *p < 0.05, versus with the control group; #p < 0.05, versus with the vehicle/WT-injury group.