FIG. 2.

Cytokines and chemokines levels in sera from experimental animals and in supernatants of primary endothelial cells isolated from WT and Lyst- (LYS) mice, treated with LPS for 24 h. The following parameters are presented: (A) interleukin (IL)-1α, (B) IL-1β, (C) IL-6, (D) IL-8, (E) granulocyte colony-stimulating factor, (F) monocyte chemotactic protein-1 (MCP1), (G) tumor necrosis factor alpha, and (H) vascular endothelial growth factor. The concentrations of cyto- and chemokines were measured using a Luminex IS100 analyzer and analyzed using appropriate curve-fitting software (Luminex 100IS, version 2.3). All results are presented as fold-increases compared to WT controls (WT-CON). Animals were injected with 5 mg/kg of LPS in PBS vehicle or vehicle only, and sacrificed after 24 h. Data are means±SEM, *p<0.05; **p<0.01 LPS versus CON; #p<0.05 LYS versus WT, n=5 per group.