FIG. 5.

(A) Representative immunoblots using cellular protein lysates from primary human umbilical vein endothelial cells (HUVEC) treated with LPS for up to 36 h. LPS-treated cells demonstrated biphasic expression profiles of (B) PMP70, (C) Pex14, and (E) p62, with an initial decrease followed by a gradual increase of protein levels compared with untreated control cells. (D) LPS induced a profound depletion of catalase followed by a slow recovery of the enzyme levels compared with untreated cells. Primary HUVEC at passages 2–5 were treated with 1 μg/ml LPS for up to 36 h. HUVEC cultured in a medium without addition of LPS served as a control. Cell lysates were separated by SDS-PAGE and analyzed by immunoblot using appropriate antibodies. Beta-tubulin served as a loading control. Data are means±SEM, *p<0.05, **p<0.01, n=4.