FIG. 6.

(A) Representative immunoblots using cellular protein lysates from HUVEC treated with 25 μM chloroquine (CHL) and stimulated with LPS for up to 36 h. LPS treatment resulted in a slow gradual decrease of (B) PMP70, (C) Pex14, and (D) catalase levels, and induced accumulation of (E) p62 compared with LPS-untreated cells. (F) Peroxisome proliferator-activated receptor-alpha (PPARα) transcription activity in LPS-stimulated HUVEC cultured with or without 25 μM CHL for up to 36 h. Primary HUVEC at passages 2–5 were pretreated 25 μM CHL for 12 h and subsequently stimulated with 1 μg/ml LPS up to 36 h (in the presence CHL). HUVEC cultured in a CHL-supplemented medium without LPS treatment served as a control. Cell lysates were separated by SDS-PAGE and analyzed by immunoblot using appropriate antibodies. Beta-tubulin served as a loading control. Data are means±SEM, *p<0.05, **p<0.01, n=4.