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. 1985 Dec;5(12):3629–3633. doi: 10.1128/mcb.5.12.3629

Phosphorylation and protein synthetic events in Xenopus laevis oocytes microinjected with pp60v-src.

J G Spivack, J L Maller
PMCID: PMC369196  PMID: 3939323

Abstract

Microinjection of purified pp60v-src, the transforming protein of Rous sarcoma virus, into Xenopus laevis oocytes accelerated the rate of progesterone- or insulin-induced meiotic maturation. This acceleration was abolished by incubating the oocytes with cycloheximide or puromycin during a 2-h interval between pp60v-src microinjection and progesterone addition. In contrast, exposure to actinomycin D did not alter the acceleration of maturation by microinjected pp60v-src. Associated with progesterone treatment and pp60v-src microinjection were a number of qualitative changes in phosphoproteins; a few of these changes are common to both stimuli. These results indicate that the action of pp60v-src in oocytes involves both phosphorylation and protein synthetic events that affect oocyte maturation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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