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R. rickettsii and cytokines induced a loss of β-catenin (a, d, g, j) and p120 (b, e, h, k) staining at intercellular junctions. Cells were infected with 10 MOI of R. rickettsii and/or stimulated with 10 ng/ml of TNF-α, IL-1β, and IFN-γ and incubated at 37°C for 24 h. Overlaid images indicate the colocalization of β-catenin and p120 (c, f, i, l). Arrowheads indicate proper localization of adherens junctions at cell–cell contacts.
