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. Author manuscript; available in PMC: 2013 Jun 25.
Published in final edited form as: Cytotherapy. 2011 May 4;13(8):976–986. doi: 10.3109/14653249.2011.575356

Figure 2.

Figure 2

Virus-specific reactivity of generated T cells by ELISPOT and intracellular cytokine staining. (a) Virus-specific activity of nine CTL lines as determined by IFN-γ ELISPOT assay in response to direct stimulation with CMV-pp65, CMV-IE-1, Ad-hexon, Ad-penton and autologous EBV LCL. For each T-cell line, mean values of duplicate or triplicate experiments are reported and error bars indicate the standard error. (b) Virus-specific activity of resulting T cells from one representative donor as measured by intracellular cytokine staining of IFN-γ after pulsing overnight with CMV-pp65, CMV-IE-1, Ad-hexon or Ad-penton pepmix. (c) A panel of pepmixes against EBV antigens (EBNA-3a, b, and c, as well as LMP1, LMP2 and BZLF-1) was tested against resulting T cells. Data are from one representative T-cell line. For each T-cell line, mean values of duplicate or triplicate experiments are reported and error bars indicate the standard deviation.