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. Author manuscript; available in PMC: 2013 Jun 25.
Published in final edited form as: Cytotherapy. 2011 May 4;13(8):976–986. doi: 10.3109/14653249.2011.575356

Figure 3.

Figure 3

Epitope mapping of CMV-IE-1, CMV-pp65 and Ad-hexon. (a, b) Ad5f35-IE-1-I-pp65 T cells were screened by ELISPOT against 11 pools of hexon peptides, 21 pools of pp65 peptides and 20 pools of IE-1 peptides (all of which contain 20-mer peptides overlapping by 15 amino acids) to identify which peptides for each antigen were recognized by each T-cell line. Two representative donors are shown. Results are expressed as the number of SFC/100 000 cells. (c) Where available, identified epitopes were confirmed by pentamer analysis for HLA-A1-, HLA-A2-, HLA-A24-, HLA-B7- and HLA-B8-restricted epitopes. Indicated is the percentage of pentamer-positive cells within the CD8+ population. Two representative donors are shown.