Table 1.

Characteristics of the D and J genes identified in 100 random IG heavy chain sequences^a

	IgBLAST	iHMMune-align
Average D gene mutations per sequence (average D gene length)	0.04 (16.29)	0.056 (17.43)
Average J gene mutations per sequence (average J gene length)	0.08 (44.29)	0.23 (44.52)

^aOne hundred IG heavy chain sequences are randomly selected from NCBI nr database (available in Supplementary File S1). The selection is based on 100% identity match to any heavy chain germline gene from IMGT database as determined by BLAST program with default parameters; therefore, there is no previous knowledge about their D and J gene compositions. Tests were performed using web IgBLAST and stand-alone iHMMune-align (version iHMMune-align_26-11-2007.zip) with default search parameters. iHMMune-align did not return a D gene match for 11 sequences that were excluded from D gene analysis.