FIG. 4.

Differential contributions of GR domains to the TGF-β superinduction. Transient transfection and treatment of M12 cells were as described for Fig. 1B. (A) Cotransfection of 3 μg of pCI vector (mock) or of pSTC-TK encoding various parts of the rat GR (see scheme in panel B) with 3 μg of pGL3-basic/wt LTR. Normalization was done with the protein content, and error bars denote the standard deviations. Note the difference in scale between the left and right portions of panel A. (B) Scheme of the GR and its segments used in panel A. Numbers to the right indicate amino acids. Positions 3 to 795 is the full-length GR. A to F above the line denote conventional divisions of the GR. Below the line are functional domains for transactivation (AF-1 and AF-2), DNA binding (DBD), and ligand binding (LBD). BSA, bovine serum albumin.