FIG. 1.

Role of integrins in Ad vector infection of bone marrow-derived DC. DC were infected with AdL.F* (CAR binding ablated), AdL.PB* (integrin binding ablated), AdL.F*PB* (CAR integrin binding base ablated), or AdL (intact CAR and integrin binding) at 10³ PU/cell. All vectors express luciferase (L) as a marker transgene. Infection with equal amounts of AdNull or uninfected cells were used as controls. Luciferase expression was measured in cell lysates 24 h following infection and was normalized to total cellular protein. Data are shown as the mean and SEM of triplicate measurements of one of three independent experiments.