TABLE 1.
Env source | Target cells | Species | Titer (AP+ FFU/ml) for indicated vector:
|
|
---|---|---|---|---|
Lentiviral | Oncoretroviral | |||
JSRV | HTX | Human | 1.1 × 104 | 4.2 × 104 |
293 | Human | 5.0 × 104 | 3.8 × 104 | |
BEAS-2B | Human | 2.9 × 104 | 2.5 × 104 | |
SSF | Sheep | 7.5 × 104 | 1.0 × 105 | |
NIH 3T3 | Mouse | <5 | <5 | |
208F | Rat | <5 | <5 | |
VSV-G | HTX | Human | 3.4 × 104 | 1.5 × 106 |
Titers of viral stocks were determined by infection of cells seeded the day before at 105 cells per well of 6-well plates with serially diluted virus, and AP+ foci were counted 3 days after infection. The values shown are the means of the results of at least two experiments, and the variation in the results of the experiments was no more than 10%.