TABLE 2.
Titers of HIV-1 lentiviral and MoMLV oncoretroviral vectors pseudotyped with chimeric JSRV envelopesa
Env constructb | Lentiviral vector titer (AP+ FFU/ml) on indicated cell type:
|
Oncoretroviral vector titer (AP+ FFU/ml) on indicated cell type:
|
||
---|---|---|---|---|
HTX | SSF | HTX | SSF | |
JSRV Env | 1.3 × 104 | 7.8 × 104 | 2.9 × 104 | 1.1 × 105 |
JSRV-HIV | 7.7 × 103 | 4.7 × 104 | 1.7 × 102 | 1.3 × 104 |
JSRV-10A1 | 8.6 × 102 | 8.0 × 103 | 1.1 × 102 | 3.7 × 102 |
JSRV-10A1R− | 1.3 × 103 | 7.0 × 103 | 2.4 × 103 | 9.2 × 103 |
The experimental approaches used were the same as those described in Table 1 except that wild-type or nontransforming JSRV Env constructs were used for pseudotyping. The values shown are the means of the results of at least two experiments, and the variation in the results of the experiments was no more than 10%.
See the legend to Fig. 1 for construct nomenclature.