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. 2004 Mar;78(5):2642–2647. doi: 10.1128/JVI.78.5.2642-2647.2003

TABLE 3.

Transduction by HIV-1 lentiviral and MoMLV oncoretroviral vectors pseudotyped with JSRV Env is restricted to cells expressing the JSRV receptor Hyal2a

Vector type Env construct Vector titer (AP+ FFU/ml) for indicated target cells:
NIH 3T3 NIH 3T3/LL2SN 208F 208F/LL2SN
Lentiviral JSRV <5 2.8 × 103 <5 1.5 × 102
JSRV-HIV <5 3.7 × 103 <5 9.0 × 102
JSRV-10A1 <5 2.5 × 102 <5 2.5 × 102
JSRV-10A1R− <5 3.0 × 102 <5 3.5 × 102
VSV-G 5.8 × 104 6.4 × 104 9.0 × 103 1.3 × 104
Oncoretroviral JSRV <5 2.8 × 105 <5 1.5 × 105
JSRV-HIV <5 2.8 × 104 <5 9.0 × 102
JSRV-10A1 <5 8.0 × 102 <5 2.5 × 102
JSRV-10A1R− <5 1.8 × 104 <5 8.5 × 103
VSV-G 1.4 × 106 1.6 × 106 8.6 × 105 8.0 × 105
a

The methods used were the same as those described in Table 1. The titers shown are the means of the results of two experiments and varied by <10%. NIH 3T3/LL2SN and 208F/LL2SN cells are NIH 3T3 and 208F cells that were transduced with a retroviral vector that expresses human Hyal2.